Automated Assay of Glycosidasesl
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چکیده
Automated methodology for monitoring the presence of one or more glycosidases in biological extracts has been described (l-7). These procedures require use of large amounts of synthetic glycoside(s) (l-4,6,7), compound automated systems (5), or extensive mechanical and/or electrical alterations of existing apparatus (8). The present paper describes a simple modification of the Technicon Basic AutoAnalyzer sampler system which allows simultaneous sampling of enzyme and substrate-buffer solutions and requires small amounts of substrate and enzyme for automated enzyme assay. The versatility of this system for the assay of glycosidases and the study of their kinetic parameters is demonstrated utilizing bovine testicular N-acetyl-P-hexosaminidase (EC 3.2.1.30).
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